Effects of paternal age on the outcome of assisted reproductive technology

Hinting, A.; Hendra; Lunardhi, H.; Pramono, H.
May 2008
Reproductive BioMedicine Online (Reproductive Healthcare Limited;May2008 Supplement 2, Vol. 16 Issue S2, pS-20
Academic Journal
Introduction: The objective of this study was to identify the effect of paternal age on the outcome of conventional IVF and intracytoplasmic sperm injection (ICSI) in regard with both fertilization and the risk of pregnancy failure. Material/Methods: This study was a retrospective study of the patients attending an assisted reproduction programme of a private fertility clinic. It involved 2327 cycles of couples, either conventional IVF or ICSI from January 1997 to December 2006. Ovulation inductions, oocyte retrievals, embryo cultures and embryo transfers were done by using standard protocols. The age of male and female partners was divided in an interval of 5 years. The estimation of fertilization rate with IVF was based on the division of cell count containing two pronuclei (2PN) and the inseminated oocytes, whereas with ICSI the rate was estimated by the division of 2PN and the count of sperm-injected oocytes. Pregnancy detection was carried out by examining serum human chorionic gonadotrophin concentration and the presence of gestational sac by using ultrasonographic examination. Data processing was undertaken using SPSS 13.0 for Windows, including descriptive data analysis, cross-tabulation between the couples' age groups, and binary logistic regression to obtain the odds ratio. Results: The mean age of male partner was 38.05 ± 6.4 years with a range of 23-74 years; while the female partner was 33.67 ± 4.7 years with a range of 18-47 year. Correlation between paternal age and fertilization rate, particularly with IVF, is statistically significant (P < 0.05), while correlation with ICSI had no significance. Binary logistic regression analysis revealed that paternal age had no significant correlation (P = 0.570) with the risk of pregnancy failure in general, either with IVF (P = 0.454) or ICSI (P = 0.879). Although the correlation model was statistically not significant, in male partners aged more than 40 years, as compared with those less than 30 years, there was a predisposing increase of odds ratio as much as 2.086 (95% CI = 1.803-2.412) in all couples attending an assisted reproduction programme. Different from those in male age group, age in female partners indicated significant correlation (P = 0.012) with the risk of pregnancy failure. The odds ratio of female partner of more than 40 years old compared with those with age less than 30 years was 4.268 (95% CI = 3.190-5.709). Conclusions: Male age has an effect on conventional IVF fertilization rate, but has no effect on ICSI. Although predisposing increase of the risk of pregnancy failure in male aged more than 40 years is apparent, statistical analysis reveals that age in male partner has no significant correlation with the risk of pregnancy failure in assisted reproduction programmes in general, either with conventional IVF or ICSI.


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