TITLE

An improved method for determination of gene copy numbers in transgenic mice by serial dilution curves obtained by real-time quantitative PCR assay

PUB. DATE
October 2005
SOURCE
Croatian Medical Journal;2005, Vol. 46 Issue 5, p863
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Presents an abstract of a study which determined gene copy numbers in transgenic mice by serial dilution curves obtained by real-time quantitative polymerase chain reaction assay.
ACCESSION #
18487268

 

Related Articles

  • Developmentally distinct effects on human ϵ-, γ- and δ-globin levels caused by the absence or altered position of the human β-globin gene in YAC transgenic mice. Bauchwitz, Robert // Human Molecular Genetics;Mar2000, Vol. 9 Issue 4, p561 

    Reports on the use of primer extension and sensitive real-time polymerase chain reaction (RT-PCR) assays to quantify the effects of beta-globin sequence modifications on globin levels in transgenic mice. Importance of the human beta-globin locus in the study of developmentally regulated...

  • TOWARD UNDERSTANDING THE FUNCTION OF AMELOGENIN USING TRANSGENIC MICE. IBARAKI-O'CONNOR, K.; NAKATA, K.; YOUNG, M. F. // Advances in Dental Research;Nov1996, Vol. 10 Issue 2, p208 

    The purpose of this study was to establish transgenic mouse lines as a tool to investigate the function of amelogenin during mineralization by causing ectopic production of amelogenin and studying its effect. The mouse amelogenin (mAme) was cloned from a 16-day-old whole mouse embryo cDNA...

  • Defining the expression pattern of the LGI1 gene in BAC transgenic mice. Head, Karen; Shiaoching Gong; Joseph, Sheldon; Cuidong Wang; Burkhardt, Tania; Rossi, Michael R.; LaDuca, Jeffrey; Sei-Ichi Matsui; Vaughan, Mary; Hicks, David G.; Heintz, Nathaniel; Cowell, John K. // Mammalian Genome;May2007, Vol. 18 Issue 5, p328 

    The LGI1 gene has been implicated in the development of epilepsy and the invasion phenotype of glial cells. Controversy over the specific tissue expression pattern of this gene has stemmed from conflicting reports generated using immunohistochemistry and the polymerase chain reaction. LGI1 is...

  • A doxycycline-inducible, tissue-specific aromatase-expressing transgenic mouse. Chow, Jenny; Price, John; Bills, Margaret; Simpson, Evan; Boon, Wah // Transgenic Research;Apr2012, Vol. 21 Issue 2, p415 

    Aromatase converts androgens to estrogens and it is expressed in gonads and non-reproductive tissues (e.g. brain and adipose tissues). As circulating levels of estrogens in males are low, we hypothesize that local estrogen production is important for the regulation of physiological functions...

  • β-Glucanase specific expression in the parotid gland of transgenic mice. Guan, Li-zheng; Sun, Yu-ping; Xi, Qian-yun; Wang, Jing-lan; Zhou, Jun-yun; Shu, Gang; Jiang, Qing-yan; Zhang, Yong-liang // Transgenic Research;Aug2013, Vol. 22 Issue 4, p805 

    The feasibility of using the pig parotid secretory protein promoter to drive the β-glucanase transgene expression in mouse parotid glands was examined in this study. The parotid gland-specific vector expressing β-glucanase gene (GLU, from Paenibacillus polymyxa CP7) was constructed....

  • Role of T-type calcium channel subunits in post-myocardial infarction remodelling probed with genetically engineered mice. Le Quang, Khai; Naud, Patrice; Qi, Xiao-Yan; Duval, Francine; Shi, Yan-Fen; Gillis, Marc-Antoine; Comtois, Philippe; Tardif, Jean-Claude; Li, Danshi; Levesque, Paul C.; Dobrev, Dobromir; Charpentier, Flavien; Nattel, Stanley // Cardiovascular Research;Aug2011, Vol. 91 Issue 3, p420 

    Aims Previous studies suggested that T-type Ca2+-current (ICaT)-blockers improve cardiac remodelling, but all available ICaT-blockers have non-specific actions on other currents and/or functions. To clarify the role of ICaT in cardiac remodelling, we studied mice with either of the principal...

  • The Construction and Expression of Lysine-Rich Gene in the Mammary Gland of Transgenic Mice. Ma, Xin; Zhang, Peng; Song, Guangqi; Chen, Yue; Wang, Zhongwei; Yin, Yupeng; Kong, Delong; Zhang, Sheng; Zhao, Zhihui; Ouyang, Hongsheng; Tang, Bo; Li, Ziyi // DNA & Cell Biology;Aug2012, Vol. 31 Issue 8, p1372 

    Lysine is the limiting amino acid in cereal grains, which represent a major source of human food and animal feed worldwide, and is considered the most important of the essential amino acids. In this study, β-casein, αS2-casein, and lactotransferrin cDNA clone fragments encoding lysine-rich...

  • Maternal behavior in transgenic mice with reduced fibroblast growth factor receptor function in gonadotropin-releasing hormone neurons. Brooks*,, Leah R.; Le, Carter Duyet V.; Chung, Wilson C.; Pei-San Tsai // Behavioral & Brain Functions;2012, Vol. 8 Issue 1, p47 

    Background: Fibroblast growth factors (FGFs) and their receptors (FGFRs) are necessary for the proper development of gonadotropin-releasing hormone (GnRH) neurons, which are key activators of the hypothalamo-pituitary-gonadal axis. Transgenic mice that have the targeted expression of a dominant...

  • Biological characteristics of cleft palate relevant gene thyroid transcription factor-2 transgenic mice. Huang Lei; Shi Bing; Zheng Qian; Meng Tian; Wang Yan // West China Journal of Stomatology;Aug2014, Vol. 32 Issue 4, p345 

    Objective The aim of this study is to establish a transgenic mouse model for cleft palate relevant gene thyroid transcription factor-2 (TTF-2), which can be used to study palatal shelf development when the expression pattern and regular activation of TTF-2 is altered. Methods The C57BL/6J mouse...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics